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KMID : 0364820200560020152
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Korean Journal of Microbiology
2020 Volume.56 No. 2 p.152 ~ p.159
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Isolation and characterization of strain Rouxiella sp. S1S-2 producing antibacterial compound
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Nam Young-Ho
Hwang Buyng-Su
Choi Ah-young
Chung Eu-Jin
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Abstract
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A Gram-negative bacterial strain S1S-2 with antibacterial activity was isolated from sediment collected from Osipcheon
in Yeongdeok-Gun. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain S1S-2 belonged to
the genus Rouxiella and shared 98.7~99.5% sequence similarity with Rouxiella species. Based on the results of API kit testing and some physiological characteristics, the strain was identified and named Rouxiella sp. S1S-2. The strain revealed antibiosis against pathogenic bacteria, including Bacillus cereus KCTC 3624, Escherichia coli KCTC 2443, and Staphylococcus aureus KCCM 40510 (methicillin-resistant strain). The effects of commercial culture media, temperature, and initial pH on the cell growth and antibacterial activity were confirmed for culture optimization of strain S1S-2. When the strain was cultured in LB, NB, TSB, R2A media, the antibacterial activity did not show. The optimal conditions for growth and antibacterial activity of strain S1S-2 were found to be YPD medium and 25°C. We also isolated the antibacterial compound from ethyl acetate fraction of the bacterial culture and its chemical structure was identified as maculosin (1) by ESI-MS, 1H-NMR, and 13C-NMR analyses. This study is the first report that newly isolated strain Rouxiella sp. S1S-2 showed antibacterial activity against pathogenic bacteria.
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KEYWORD
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Rouxiella sp., antibacterial activity, maculosin, optimal culture condition
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